東京工科大学 研究報告等

今井 伸二郎
所属  応用生物学部 応用生物学科
職種  教授
言語種別
英語
発行・発表の年月
2017年03月
形態種別
学術論文
査読
査読あり
標題
Alkylresorcinols activate SIRT1 and delay ageing in Drosophila melanogaster.
執筆形態
共著
掲載誌名
Scientific Reports
出版社・発行元
Nature Publishing Group
巻・号・頁
7,43679
著者・共著者
Kayashima Y, Katayanagi Y, Tanaka K, Fukutomi R, Hiramoto S, Imai S.
概要
Sirtuins are enzymes that catalyze NAD+ dependent protein deacetylation. The natural polyphenolic compound resveratrol received renewed interest when recent findings implicated resveratrol as a potent SIRT1 activator capable of mimicking the effects of calorie restriction. However, resveratrol directly interacts with fluorophore-containing peptide substrates. It was demonstrated that the SIRT1 activation of resveratrol is affected by the amino acid composition of the substrate. Resveratrol did increase the enzyme activity in cases in which hydrophobic amino acids are at the +1 position to the acetylated lysine in the substrate. Alkylresorcinols (ARs) are compounds that belong to the family of phenolic lipids, and they are found in numerous biological species. Here we show that the natural activators ARs increased the Vmax of recombinant SIRT1 for NAD+ and peptide substrate, and that ARs decreased acetylated histone in human monocyte cells by stimulating SIRT1-dependent deacetylation of substrates. ARs also extended the lifespan of Drosophila melanogaster, which was shown to be dependent on functional Sir2. Our results demonstrated that ARs are natural catalytic activators for sirtuin.
論文(査読付)ファイル