ヨシダ　マサキ   Masaki YOSHIDA   吉田　雅紀    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2022/10
形態種別	学術論文
査読	査読あり
標題	Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers
執筆形態	共著
掲載誌名	J Dermatol Sci
掲載区分	国外
出版社・発行元	Wiley
巻・号・頁	108(1),pp.22-29
著者・共著者	Yushi Katsuyama, Yumiko Yamawaki, Yuki Sato, Sora Muraoka, Masaki Yoshida, Yuri Okano, Hitoshi Masaki
概要	Background Decreases of collagen fibers and the disappearance of oxytalan fibers are typical symptoms of photoaged skin. Although a low quality of mitochondria (MT) in photoaged skin cells has been observed, it is unknown whether the decreased quality of MT is responsible for the insufficient formation of dermal fibers. Objective To identify the role of mitochondrial quality in skin photoaging focusing on the formation of dermal fibers. Methods Type I collagen and fibrillin-1 fibers in normal human dermal fibroblasts (NHDFs) were observed by immunostaining. Type I collagen and fibrillin-1 proteins in NHDFs were quantified by ELISA. Mitochondrial quality was evaluated by measuring levels of intracellular ATP and MITOL, which regulate mitochondrial quality. Results UVA-irradiated NHDFs formed insufficient type I collagen and fibrillin-1 fibers and had a decreased ratio of extracellular versus intracellular levels of those proteins. Although expression levels of motor proteins that transport those proteins intracellularly were not affected by UVA, intracellular ATP levels, which is the driving force of motor proteins, were decreased by UVA along with decreased MITOL protein. Knockdown of MITOL in NHDFs decreased the level of intracellular ATP and caused the insufficient formation of type I collagen and fibrillin-1 fibers due to interfering with the secretion of those proteins.