マツイ　タケシ   Takeshi Matsui   松井　毅    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2004/08
形態種別	学術論文
査読	査読あり
標題	Identification of novel keratinocyte-secreted peptides dermokine-α/-β and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1
執筆形態	共著
掲載誌名	Genomics
掲載区分	国外
出版社・発行元	ELSEVIER
巻・号・頁	84(2),pp.384-397
担当区分	筆頭著者
著者・共著者	Takeshi Matsui, Fumie Hayashi-Kisumi, Yoko Kinoshita, Sayaka Katahira, Kazumasa Morita, Yoshiki Miyachi, Yuichi Ono, Toshio Imai, Yoko Tanigawa, Tohru Komiya, Shoichiro Tsukita
概要	We performed high-throughput in situ hybridization screening of sections of mouse epidermis using an equalized skin cDNA library as probes and identified a novel gene giving rise to two splicing variants, both of which are expressed in the spinous layer. This gene was mapped between two genes encoding keratinocyte-related peptides, suprabasin and keratinocyte differentiation-associated protein (Kdap), on human chromosome 19q13.1. These gene products appeared to carry functional signal sequences. We then designated these two splicing variants as dermokine-a and -h. Northern blotting and quantitative RT-PCR revealed that dermokine-alpha/-beta, suprabasin, and Kdap were highly expressed in stratified epithelia. In mouse embryonic development, dermokine-alpha/-beta began to be expressed during the period of stratification. Also, in differentiating primary cultured human keratinocytes, transcription of dermokine-alpha/-beta, suprabasin, and Kdap was induced. These findings indicated that dermokine-alpha/-beta, suprabasin, and Kdap are secreted from the spinous layer of the stratified epithelia and that these genes form a novel gene complex on the chromosome.
外部リンクURL	https://www.sciencedirect.com/science/article/abs/pii/S0888754304000874?via%3Dihub