アラカワ　タカヒロ   荒川　貴博    所属   工学部 電気電子工学科    職種   准教授
言語種別	英語
発行・発表の年月	2022/10
形態種別	学術論文
査読	査読あり
標題	Enzyme-embedded electrospun fiber sensor of hydrophilic polymer for fluorometric ethanol gas imaging in vapor phase
執筆形態	共著
掲載誌名	Biosensors and Bioelectronics
掲載区分	国外
出版社・発行元	Elsevier
巻・号・頁	114453
総ページ数	8
著者・共著者	Iitani K, Nakaya M, Tomono T, Toma K, Arakawa T, Tsuchido Y, Mitsubayashi K, Takeda N
概要	Non-invasive measurement of volatile organic compounds (VOCs) emitted from living organisms is a powerful technique for diagnosing health conditions or diseases in humans. Bio-based gas sensors are suitable for the sensitive and selective measurement of a target VOC from a complex mixture of VOCs. Conventional bio-based sensors are normally prepared as wet-type probes to maintain proteins such as enzymes in a stable state, resulting in limitations in the commercialization of sensors, their operating environment, and performance. In this study, we present an enzyme-based fluorometric electrospun fiber sensor (eFES) mesh as a gas-phase biosensor in dry form. The eFES mesh targeting ethanol was fabricated by simple one-step electrospinning of polyvinyl alcohol with an alcohol dehydrogenase and an oxidized form of nicotinamide adenine dinucleotide. The enzyme embedded in the eFES mesh worked actively in a dry state without pretreatment. Substrate specificity was also maintained, and the sensor responded well to ethanol with a sufficient dynamic range. Adjustment of the pH and coenzyme quantity in the eFES mesh also affected enzyme activity. The dry-form biosensor—eFES mesh—will open a new direction for gas-phase biosensors because of its remarkable performance and simple fabrication, which is advantageous for commercialization.
DOI	https://doi.org/10.1016/j.bios.2022.114453
researchmap用URL	https://doi.org/10.1016/j.bios.2022.114453
外部リンクURL	https://doi.org/10.1016/j.bios.2022.114453