アラカワ　タカヒロ   荒川　貴博    所属   工学部 電気電子工学科    職種   准教授
言語種別	英語
発行・発表の年月	2022/10
形態種別	学術論文
査読	査読あり
標題	Blood sorbitol measurement in diabetic rats treated with an aldose reductase inhibitor using an improved fiber-optic sorbitol biosensor
執筆形態	共著
掲載誌名	Talanta
掲載区分	国外
出版社・発行元	Elsevier
巻・号・頁	123595
総ページ数	7
著者・共著者	Gessei T, Monkawa A, Arakawa T, Mitsubayashi K
概要	Sorbitol is known as a biomarker for the evaluation of the progress of diabetic complications. We have developed a sorbitol biosensor using an optical fiber for rapid diagnosis and pathological evaluation of diabetic complications. In this paper, we measured blood sorbitol in diabetic rats using an improved biosensor, and discussed the effectiveness of the developed biosensor and the significance of sorbitol measurement. In order to investigate the effectiveness of the developed biosensor, the blood sorbitol level of type II diabetic rats prepared by streptozotocin administration was measured with the developed sensor. The values of sorbitol were highly correlated with the values measured by the F-kit of food analysis and that we confirmed the sorbitol concentration could be quantified using the developed biosensor. Furthermore, the aldose reductase inhibitor “eparlrestat”, which is a therapeutic drug that suppresses the accumulation of sorbitol, was administered to diabetic rats, and the blood sorbitol level was measured with the developed biosensor. As a result, the blood glucose level was high in both the treated group and the non-treated group, but the blood sorbitol level in the treated group decreased. The results suggest that the measurement of the sorbitol level with the developed biosensor in addition to the blood glucose level enables evaluation of complications like diabetic neuropathy. In the future, we expected that the developed sorbitol biosensor will be miniaturized, the pretreatment method for blood samples will be simplified, and it will be applied to the development of therapeutic agents for diabetic complications and personalized medicine.
DOI	https://doi.org/10.1016/j.talanta.2022.123595
researchmap用URL	https://doi.org/10.1016/j.talanta.2022.123595
外部リンクURL	https://doi.org/10.1016/j.talanta.2022.123595