カシバ　ミサト   加柴　美里    所属   学環 教養学環    職種   教授
言語種別	英語
発行・発表の年月	2022/09
形態種別	学術論文
査読	査読あり
標題	Cellular level of coenzyme Q increases with neuronal differentiation, playing an important role in neural elongations.
執筆形態	共著
掲載誌名	Journal of Clinical Biochemistry and Nutrition
掲載区分	国内
出版社・発行元	JCBN
巻・号・頁	71(2),pp.89-96
担当区分	最終著者,責任著者
著者・共著者	Akari Nakamura, Mizuho Okamoto, Ayaka Maeda, Huiyu Jiang, Kyosuke Sugawara, Kanae Kitatani, Susumu Takekoshi , Akio Fujisawa, Yorihiro Yamamoto, Misato Kashiba
概要	Deficiency of coenzyme Q has been reported in various neurological diseases, and the behavior of this lipid in neurons has attracted attention. However, the behavior of this lipid in normal neurons remains unclear. In this study, we analyzed the concentration of coenzyme Q before and after neuronal differentiation. Nerve growth factor treatment of PC12 cells caused neurite outgrowth and neuronal differentiation, and the amount of intracellular coenzyme Q increased dramatically during this process. In addition, when the serum was removed from the culture medium of N1E-115 cells and the neurite outgrowth was confirmed, the intracellular coenzyme Q level also increased. To elucidate the role of the increased coenzyme Q, we administered NGF to PC12 cells with coenzyme Q synthesis inhibitors and found that coenzyme Q levels decreased, neurite outgrowth was impaired, and differentiation markers were reduced. These results indicate that coenzyme Q levels increase during neuronal differentiation and that this increase is important for neurite outgrowth.