ナカムラ ヒデアキ   Hideaki Nakamura
  中村 秀明
   所属   学環 教養学環
   職種   専任講師
言語種別 英語
発行・発表の年月 2007/12
形態種別 学術論文
査読 査読あり
標題 Development of a package-free transparent disposable biosensor chip for simultaneous measurements of blood constituents and investigation of its storage stability (Cited by 8)
執筆形態 共著
掲載誌名 Biosensors and Bioelectronics
出版社・発行元 Elsevier B.V. (Netherlands)
巻・号・頁 23(5),pp.621-626
総ページ数 6
担当範囲 All of the manuscript
著者・共著者 H. Nakamura*, K. Tohyama, M. Tanaka, S. Shinohara, Y. Tokunaga, F. Kurusu, S. Koide, M. Gotoh, and I. Karube*
概要 A package-free transparent disposable biosensor chip was developed by a screen-printing technique. The biosensor chip was fabricated by stacking a substrate with two carbon electrodes on its surface, a spacer consisting of a resist layer and an adhesive layer, and a cover. The structure of the chip keeps the interior of the reaction-detecting section airtight until use. The chip is equipped with double electrochemical measuring elements for the simultaneous measurement of multiple items, and the reagent layer was developed in sample-feeding path. The sample-inlet port and air-discharge port are simultaneously opened by longitudinally folding in two biosensor units with a notch as a boundary. Then the shape of the chip is changed to a V-shape. The reaction-detecting section of the chip has a 1.0 μl sample volume for one biosensor unit. Excellent results were obtained with the chip in initial simultaneous chronoamperometric measurements of both glucose (r = 1.00) and lactate (r = 0.998) in the same samples. The stability of the enzyme sensor signals of the chip was estimated at ambient atmosphere on 8 testing days during a 6-month period. The results were compared with those obtained for an unpackaged chip used as a control. The package-free chip proved to be twice as good as the control chip in terms of the reproducibility of slopes from 16 calibration curves (one calibration curve: 0, 100, 300, 500 mg dl−1 glucose; n = 3) and 4.6 times better in terms of the reproducibility of correlation coefficients from the 16 calibration curves.

Keywords
Package free; V-shaped transparent disposable biosensor chip; Simultaneous measurements; Blood constituents; SMBG; Glucose
DOI doi:10.1016/j.bios.2007.07.006
ISSN 0956-5663
論文(査読付)ファイル DOWNLOAD
外部リンクURL http://www.sciencedirect.com/science/article/pii/S0956566307002977