カトウ　テル   加藤　輝    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2013/11
形態種別	国際会議論文
査読	査読あり
標題	A novel real-time PCR assay for the pinpoint detection of methylated DNA
執筆形態	共著
掲載区分	国外
出版社・発行元	The 40th international Symposium on Nucleic Acids Chemistry Abstracts
著者・共著者	Kenta Takanashi
概要	Methylated DNA plays an important role in the epigenetic gene regulation and thus can be a promising biomarker for cancer diagnosis. Therefore, a simple method for the analysis of methylation ratio of cytosines in target gene is highly required. We herein report a new method for the pinpoint detection of 5-methylcytosine that is based on the selective chemical modification of cytosine at the branching point of DNA three-way junction and the real-time PCR assay.