サトウ　アツシ   佐藤　淳    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2016/03
形態種別	学術論文
査読	査読あり
標題	Soluble human intestinal lactoferrin receptor: Ca2+-dependent binding to Sepharose-based matrices
執筆形態	共著
掲載誌名	Biological & Pharmaceutical Bulletin
掲載区分	国内
出版社・発行元	The Pharmaceutical Society of Japan
巻・号・頁	39(3),pp.435-439
著者・共著者	Yuta Oshima, Kohei Seki, Masataka Shibuya, Yuki Naka, Tatsuya Yokoyama, Atsushi Sato
概要	A soluble form of human intestinal lactoferrin receptor (shLFR) is identical to human intelectin-1 (hITLN-1), a galactofuranose-binding protein that acts as a host defense against invading pathogenic microorganisms. We found that recombinant shLFR, expressed in mammalian cells (CHO DG44, COS-1, and RK13), binds tightly to Sepharose 4 Fast Flow (FF)-based matrices in a Ca(2+)-dependent manner. This binding of shLFR to Sepharose 4 FF-based matrices was inhibited by excess D-galactose, but not by D-glucose, suggesting that shLFR recognizes repeating units of α-1,6-linked D-galactose in Sepharose 4 FF. Furthermore, shLFR could bind to both Sepharose 4B- and Sepharose 6B-based matrices that were not crosslinked in a similar manner as to Sepharose 4 FF-based matrices. Therefore, shLFR (hITLN-1) binds to Sepharose-based matrices in a Ca(2+)-dependent manner. This binding property is most likely related to the ability, as host defense lectins, to recognize sepharose (agarobiose)-like structures present on the surface of invading pathogenic microorganisms.
外部リンクURL	https://www.jstage.jst.go.jp/article/bpb/39/3/39_b15-00643/_article