ヒナタ　ナエ   日向　奈惠    所属   医療保健学部 臨床工学科    職種   教授
言語種別	英語
発行・発表の年月	2022/09
形態種別	学術論文
査読	査読あり
標題	Multiple cystic sphere formation from PK-8 cells in three-dimensional culture
執筆形態	共著
掲載誌名	Biochemistry and Biophysics Reports
掲載区分	国外
出版社・発行元	Biochemistry and Biophysics Reports 2022 Sep 7;32:101339.
総ページ数	6
著者・共著者	Yuuki Shichi , Fujiya Gomi , Yoshibumi Ueda , Keisuke Nonaka , Fumio Hasegawa , Yasuko Hasegawa , Nae Hinata , Hisashi Yoshimura , Masami Yamamoto , Kimimasa Takahashi , Tomio Arai , Toshiyuki Ishiwata
概要	Three-dimensional (3D) culture of cancer cells mimics the in vivo environment. Recently, we reported that pancreatic ductal adenocarcinoma (PDAC) cell lines with epithelial and mesenchymal features formed differently shaped spheres in 3D culture. However, only PK-8 cells, the epithelial PDAC cell line with the highest E-cadherin expression among the eight PDAC cell lines, formed multiple cystic spheres in 3D culture. Optical coherence tomography revealed interconnected cysts inside the spheres. A weak inter-cellular adhesion, individual cell degeneration, necrosis, and secretory granules in the cytoplasm were observed in the PK-8 spheres using electron microscopy. The expression of MUC1, MUC5AC, and amylase was increased in PK-8 cells in the 3D culture compared with that in 2D culture. These findings suggest that highly E-cadherin-expressing epithelial PK-8 cells form multiple cystic spheres, which may be promoted by enhanced mucin and amylase synthesis in 3D culture.