イリタニ　コウヘイ   Iritani Kohei   入谷　康平    所属   工学部 応用化学科    職種   助教
言語種別	英語
発行・発表の年月	2023/07
形態種別	学術論文
査読	査読あり
標題	Identification of Cell-Attachment Factors Derived from Green Algal Cells Disrupted by Sonication in Fabrication of Cell Plastics
執筆形態	共著
掲載誌名	Bioengineering
掲載区分	国外
出版社・発行元	MDPI
巻・号・頁	10,pp.893
総ページ数	18
著者・共著者	Akihito Nakanishi, Shintaro Nemoto, Naotake Yamamoto, Kohei Iritani, Marina Watanabe
概要	Cell plastics which are composed of unicellular green algal cells have been proposed in previous studies. While unicellular green algae can be freely arranged using fabrication processes, a matrix is required to attach the cells together. To date, although the cell contents collected from Chlamydomonas reinhardtii show the possibility of attaching cells, but it is unclear which components can be considered attachment factors. Therefore, in this study, C. reinhardtii cells were disrupted with sonication, and the components were separated and purified with hexane. The cell plastics with only 0.5 wt% of intermediate showed similar mechanical properties to those with 17 wt% and 25 wt% of cell components that were untreated with hexane, meaning that the purified intermediates could function as matrices. The purified intermediate was composed of approximately 60 wt% of protein as the main component, and proteomic analysis was performed to survey the main proteins that remained after hexane treatment. The protein compositions of the cell content and purified intermediate were compared via proteomic analysis, revealing that the existing ratios of 532 proteins were increased in the purified intermediate rather than in the cell content. In particular, the outer structure of each of the 49 proteins—the intensity of which was increased by over 10 times—had characteristically random coil conformations, containing ratios of proline and alanine. The information could suggest a matrix of cell plastics, inspiring the possibility to endow the cell plastics with more properties and functions.