カシバ　ミサト   加柴　美里    所属   学環 教養学環    職種   教授
言語種別	英語
発行・発表の年月	2024/03
形態種別	学術論文
査読	査読あり
標題	Prosaposin is a novel coenzyme Q10-binding protein.
執筆形態	共著
掲載誌名	J Clin Biochem Nutr.
掲載区分	国外
出版社・発行元	J Clin Biochem Nutr
巻・号・頁	74(2),pp.108-112
担当区分	最終著者,責任著者
著者・共著者	Hasegawa M, Yamamoto Y, Fujisawa A, Kashiba M.
概要	Coenzyme Q10 (CoQ10) is essential for mitochondrial ATP production and functions as an important antioxidant in every biomembrane and lipoprotein. Due to its hydrophobicity, a binding and transfer protein for CoQ10 is plausible, and we previously described saposin B as a CoQ10-binding and transfer protein. Here, we report that prosaposin, the precursor of saposin B, also binds CoQ10. As prosaposin is both a secretory protein and integral membrane protein, it is ubiquitous in the body. Prosaposin was isolated from human seminal plasma, and CoQ10 was extracted from hexane solution into the water phase. It was additionally found that immunoprecipitates of mouse brain cytosol generated using two different anti-prosaposin antibodies contained coenzyme Q9. Furthermore, mouse liver cytosol and mouse kidney cytosol also contained prosaposin-coenzyme Q9 complex. These results suggest that prosaposin binds CoQ10 in human cells and body fluids. The significance and role of the Psap-CoQ10 complex in vivo is also discussed.