マツイ　タケシ   Takeshi Matsui   松井　毅    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2024/05
形態種別	学術論文
査読	査読あり
標題	Three stepwise pH progressions in stratum corneum for homeostatic maintenance of the skin
執筆形態	共著
掲載誌名	Nature Communications
掲載区分	国外
出版社・発行元	Springer Nature
巻・号・頁	15
国際共著	国際共著
著者・共著者	Fukuda K, Ito Y, Furuichi Y, Matsui T, Horikawa H, Miyano T, Okada T, Logtestijn V. M, Tanaka J. R, Miyawaki A, Amagai M
概要	The stratum corneum is the outermost skin layer with a vital role in skin barrier function. It is comprised of dead keratinocytes (corneocytes) and is known to maintain its thickness by shedding cells, although, the precise mechanisms that safeguard stratum corneum maturation and homeostasis remain unclear. Previous ex vivo studies have suggested a neutral-to-acidic pH gradient in the stratum corneum. Here, we use intravital pH imaging at single-corneocyte resolution to demonstrate that corneocytes actually undergo differentiation to develop three distinct zones in the stratum corneum, each with a distinct pH value. We identified a moderately acidic lower, an acidic middle, and a pH-neutral upper layer in the stratum corneum, with tight junctions playing a key role in their development. The upper pH neutral zone can adjust its pH according to the external environment and has a neutral pH under steady-state conditions owing to the influence of skin microbiota. The middle acidic pH zone provides a defensive barrier against pathogens. With mathematical modeling, we demonstrate the controlled protease activation of kallikrein-related peptidases on the stratum corneum surface that results in proper corneocyte shedding in desquamation. This work adds crucial information to our understanding of how stratum corneum homeostasis is maintained.
researchmap用URL	https://www.nature.com/articles/s41467-024-48226-z#auth-Keitaro-Fukuda-Aff1-Aff2
外部リンクURL	https://www.nature.com/articles/s41467-024-48226-z#auth-Keitaro-Fukuda-Aff1-Aff2