マツイ　トオル   松井　徹    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2021/12
形態種別	学術論文
査読	査読あり
標題	Alkane biodegradation under saline conditions.
執筆形態	共著
掲載誌名	Exprolatory Environmental Science Research
掲載区分	国外
出版社・発行元	Knowledge Empowerment Foundation
巻・号・頁	2(1),pp.127-132
総ページ数	6
担当区分	筆頭著者,責任著者
国際共著	国際共著
著者・共著者	Toru Matsui, Miho Asano, Leila El-Bassi
概要	Halophiles have a great potential in value-added compounds production such as ectoine, and use for bioremediation in hypersaline environments. As well as the halophiles, halo-tolerant microbes could be used for the similar application. In addition, screening and maintenance of such microbes could contribute in constructing a namely, ‘Bioresource Library’ for achieving the sustainable development goals (SDGs). Here, we present the characterization of halophilic-, and halotolerant bacteria, isolated from Okinawa and Hachioji, Japan, and Tunisia. Using enrichment culture and direct isolation from environmental samples in Tunisia and Okinawa, Japan, totally 100 strains were obtained as moderately halophilic bacteria. For the practical application in marine petroleum remediation, enrichment culture with a mid-chain alkane as the sole carbon source under saline condition were further conducted using soils from Tunisia and Japan (Hachioji, Tokyo). All the isolates exhibited a similar growth rate below 15 % of NaCl suggesting they are halo-tolerant bacteria. Among them, Sinomicrobium sp. belonging to CFB group was included as halo-tolerant alkane degrading bacteria from Tunisian soil, suggesting an unique gene resources for alkane degradation. For the genes related for alkane degradation, both of alk B and P450 (CYP153A) genes were detected from the isolates.