マツイ　タケシ   Takeshi Matsui   松井　毅    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2022/05
形態種別	学術論文
査読	査読あり
標題	Novel electron microscopic staining method using traditional dye, hematoxylin
執筆形態	共著
掲載誌名	Scientific Reports
掲載区分	国外
出版社・発行元	Nature Publishing Group
巻・号・頁	12,pp.7756
総ページ数	7
担当区分	責任著者
著者・共著者	Hiroyuki Sasaki, Hisako Arai, Emi Kikuchi, Hideki Saito, Keiko Seki, Takeshi Matsui
概要	Uranyl acetate (UA) has been routinely used as a staining solution for ultrathin sections used in biological electron microscopy. As a radioactive nuclear material, UA is subject to strict international regulations. To develop an alternative and easy-to-use staining method for ultrathin sections, we examined various commercial light microscopic dyes. We found that Mayer’s hematoxylin followed by Reynold’s lead citrate solution showed staining results comparable to UA and Reynold’s lead citrate solution, and this method is therefore suggested as a reliable and promising alternative to UA staining.
researchmap用URL	https://www.nature.com/articles/s41598-022-11523-y%20