ニシノ　トモヒコ   西野　智彦    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2016/12
形態種別	学術論文
査読	査読あり
標題	Transposon-mediated random gene disruption with moderate halophilic bacteria and its application for halophilic bacterial siderophore analysis
執筆形態	共著
掲載誌名	Journal of Basic Microbiology
掲載区分	国外
出版社・発行元	John Wiley & Sons, Inc
巻・号・頁	56(12),pp.1354-1359
著者・共著者	Toru Matsui and Tomohiko Nishino
概要	Analytical conditions using chromo azurol S was validated for quantification of siderophore in aqueous samples, followed by the characterization of siderophore derived from newly isolated moderately halophilic bacteria. Conditions with good linearity between the absorbance and the siderophore concentration were obtained at a siderophore concentration less than 20 µM, in the wavelength range between 630 and 660 nm with developing time for at least 2 h. Of the halophilic bacteria isolated from Tunisian soil, Halomonas sp., namely strain 21a was selected as siderophore producing halophiles.The strain produced siderophore significantly in the absence of iron in minimal medium.Siderophore-deficient mutant was obtained from gene disruptant library constructed using transposon complex by electroporation. Genomic sequence analysis of the mutant IIa10 revealed that the transposon-inserted gene was TonB-dependent receptor.