フジサワ　アキオ   藤沢　章雄    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2024/03
形態種別	学術論文
査読	査読あり
標題	5-N-Carboxyimino-6-aminopyrimidine-2,4(3H)-dione, a novel indicator for hypochlorite formation.
執筆形態	共著
掲載誌名	Journal of Clinical Biochemistry and Nutrition
掲載区分	国外
出版社・発行元	Society of Free Radical Reseach Japan
巻・号・頁	74(2),pp.113-118
総ページ数	6
担当区分	責任著者
著者・共著者	Matsubara A, Tanno H, Amekura S, Iida S, Yamamoto Y, Fujisawa A.
概要	Uric acid is an adequate and endogenous probe for identifying reactive oxygen or nitrogen species generated in vivo because its oxidation products are specific to reacted reactive oxygen or nitrogen species. Recently, we identified 5-N-carboxyimino-6-N-chloroaminopyrimidine-2,4(3H)-dione as a hypochlorite-specific oxidation product. 5-N-carboxyimino-6-N-chloroaminopyrimidine-2,4(3H)-dione was anticipated to be a biomarker for hypochlorite production in vivo. However, while it was stable in aqueous solution at weak acidic and alkaline pH (6.0-8.0), it was unstable in human plasma. In this study, we found that 5-N-carboxyimino-6-N-chloroaminopyrimidine-2,4(3H)-dione rapidly reacted with thiol compounds such as cysteine and glutathione to yield 5-N-carboxyimino-6-aminopyrimidine-2,4(3H)-dione, which was stable in human plasma unlike 5-N-carboxyimino-6-N-chloroaminopyrimidine-2,4(3H)-dione. 5-N-carboxyimino-6-aminopyrimidine-2,4(3H)-dione was produced upon uric acid degradation during myeloperoxidase-induced uric acid oxidation and lipopolysaccharide-induced pseudo-inflammation in collected 2,4(3H)-dione has potential as a marker for hypochlorite production in vivo.