キムラ　マサヒロ   木村　将大    所属   応用生物学部 応用生物学科    職種   助教
言語種別	英語
発行・発表の年月	2025/01
形態種別	学術論文
査読	査読あり
標題	Hyperactivation of human acidic chitinase (Chia) for potential medical use
執筆形態	共著
掲載誌名	Journal of Biological Chemistry
掲載区分	国外
出版社・発行元	Elsevier社
国際共著	国際共著
著者・共著者	Okawa K, Kijima M, Ishii M, Maeda N, Yasumura Y, Sakaguchi M, Kimura M, Uehara M, Tabata E, Bauer PO, Oyama F.
概要	Abstract Accumulation of environmental chitin in the lungs can lead to pulmonary fibrosis, characterized by inflammatory infiltration and fibrosis in acidic chitinase (Chia)-deficient mice. Transgenic expression of Chia in these mice ameliorated the symptoms, indicating the potential of enzyme supplementation as a promising therapeutic strategy for related lung diseases. This study focuses on utilizing hyperactivated human Chia, which exhibits low activity. We achieved significant activation of human Chia by incorporating nine amino acids derived from the crab-eating monkey (Macaca fascicularis) Chia, known for its robust chitin-degrading activity. The modified human Chia retained high activity across a broad pH spectrum and exhibited enhanced thermal stability. The amino acid substitutions associated with hyperactivation of human Chia activity occurred species specifically in monkey Chia. This discovery highlights the potential of hyperactivated Chia in treating pulmonary diseases resulting from chitin accumulation in human lungs.
論文（査読付）ファイル	DOWNLOAD
外部リンクURL	https://pmc.ncbi.nlm.nih.gov/articles/PMC11773036/