ゴトウ　サキ   後藤　早希    所属   応用生物学部 応用生物学科    職種   助教
言語種別	英語
発行・発表の年月	2018/09
形態種別	学術論文
査読	査読あり
標題	Cloning and heterologous expression of the lactate dehydrogenase genes from acid-tolerant Lactobacillus acetotolerans HT
執筆形態	共著
掲載誌名	Food Science and Technology Research
掲載区分	国外
出版社・発行元	Japanese Society for Food Science and Technology
巻・号・頁	24(5),pp.861-868
担当区分	筆頭著者
著者・共著者	Saki Goto, Akane Motomura, Ai Kawahara, Hideki Shiratsuchi, Kenji Tanaka and Hiromi Matsusaki
概要	In the acid-tolerant lactic acid bacterium Lactobacillus acetotolerans HT, genes encoding putative D- and L-lactate dehydrogenases (D-LDH, L-LDH1, and L-LDH2) were identified. To investigate their functions, heterologous expression of these LDH genes was performed using Escherichia coli DH5α. As a result, recombinant E. coli DH5α strains harboring the ldhD or ldhL1 genes produced D-lactic acid (D-LA) and L-lactic acid (L-LA), respectively. In contrast, the strain harboring the ldhL2 gene did not produce L-LA, suggesting that the translational product of ldhL2 does not exhibit L-LDH activity in vivo.
DOI	10.3136/fstr.24.861
外部リンクURL	https://doi.org/10.3136/fstr.24.861