ヨシダ　マサキ   Masaki YOSHIDA   吉田　雅紀    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2023/03
形態種別	学術論文
査読	査読あり
標題	The epidermal environment’s influence on the dermal environment in response to external stress
執筆形態	共著
掲載誌名	Skin Pharmacology
掲載区分	国外
担当区分	筆頭著者,責任著者
国際共著	国際共著
著者・共著者	Masaki Yoshida, Kyong-Oh Shin, Sora Muraoka, Yerim Choi, Jae-Ho Park, Soo-Hyun Park, Jin-Taek Hwang, Kyungho Park, Yoshikazu Uchida
概要	The outermost layer of the skin, the epidermis, is directly exposed to external stress (e.g., irradiation, allergens and chemicals). Changes in epidermal conditions/environment in response to this stress could also influence conditions of the dermis, located directly beneath the epidermis. Yet, whether/how any epidermal environment changes in response to external stress affect dermal functions has not been completely clarified. Using ultraviolet irradiation B (UVB) (which does not reach the dermis) as a model of external stress, we demonstrated some changes in epidermal conditions that did affect dermal function. Inflammatory-cytokine and -lipid mediator production were increased in cultured human keratinocytes treated with UVB, while matrix metalloproteases (MMP)-1, -3 and -9 production was increased in cultured human dermal fibroblasts incubated with a conditioned medium of keratinocytes exposed to UVB (UVB-keratinocyte-M). mRNA expression of COL1A1 (that codes Type 1 collagen) levels was decreased in fibroblasts incubated with UVB-keratinocyte-M. We next treated ex vivo organ-cultured human skin with UVB. An immunofluorescence study showed both MMP-1 and MMP-9 expression increased in both epidermis and dermis, and increased dermal collagen degradation confirmed that changes in epidermal conditions promoted this dermal MMP expression. Finally, increased MMP production and collagen degradation were attenuated by application of an agent cocktail (dihydroceramide, niacin amide, resveratrol, glucosyl hesperidin and phytosterol ester) that has been shown to improve skin barrier integrity. Taken together, epidermal environment/conditions change in response to external stress, affecting dermal conditions as well, and these negative effects of external stress on various skin layers can be pharmacologically mitigated.