フジサワ　アキオ   藤沢　章雄    所属   応用生物学部 応用生物学科    職種   教授
言語種別	日本語
発行・発表の年月	2021/08
形態種別	学術論文
査読	査読あり
標題	Differentiation of THP-1 monocytes to macrophages increased mitochondrial DNA copy number but did not increase expression of mitochondrial respiratory proteins or mitochondrial transcription factor A.
執筆形態	共著
掲載誌名	Archives of Biochemistry and Biophysics
掲載区分	国外
出版社・発行元	Archives of Biochemistry and Biophysics, Elsevier
巻・号・頁	710
著者・共著者	Okamoto M, Shimogishi M, Nakamura A, Suga Y, Sugawara K, Sato M, Nishi R, Fujisawa A, Yamamoto Y, Kashiba M.
概要	Monocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of monocytes. Although PMA treatment increased mtDNAcn, the expression levels of mRNAs encoded in mtDNA were decreased. The levels of mitochondrial transcription factor A mRNA and protein were also decreased. The levels of coenzyme Q10 remained unchanged. These results imply that, although mtDNAcn is considered as a health marker, the levels of mtDNAcn may not always be consistent with the parameters of mitochondrial functions.