ミカミ　アカネ   三上　あかね    所属   医療保健学部 臨床検査学科    職種   専任講師
言語種別	英語
発行・発表の年月	2020/02
形態種別	学術論文
査読	査読あり
標題	A novel biofunctionalizing peptide for metallic alloy
執筆形態	共著
掲載誌名	Biotechnology letter
掲載区分	国外
出版社・発行元	Springer
巻・号・頁	42,pp.747-756
総ページ数	10
著者・共著者	Akane Sakaguchi-Mikami, Kazuhiro Fujimoto, Tetsushi Taguchi, Isao　Karube，Tomohiko Yamazaki
概要	Objectives Improving biocompatibility of metallic alloy biomaterials has been of great interest to prevent implant associated-diseases, such as stent thrombosis. Herein a simple and efficient procedure was designed to biofunctionalize a biomaterial surface by isolating a SUS316L stainless steel binding peptide. Results After three rounds of phage panning procedure, 12 mer peptide (SBP-A; VQHNTKYSVVIR) was identified as SUS316L-binding peptide. The SBP-A peptide formed a stable bond to a SUS316L modified surface and was not toxic to HUVECs. The SBP-A was then used for anti-ICAM antibody modification on SUS316L to construct a vascular endothelial cell-selective surface. The constructed surface dominantly immobilized vascular endothelial cells to smooth muscle cells, demonstrating that the SBP-A enabled simple immobilization of biomolecules without disturbing their active biological function. Conclusions The SUS316L surface was successfully biofunctionalized using the novel isolated peptide SBP-A, showing its potential as an ideal interface molecule for stent modification. This is the first report of material binding peptide-based optimal surface functionalization to promote endothelialisation. This simple and efficient biofunctionalization procedure is expected to contribute to the development of biocompatible materials.