イリタニ　コウヘイ   Iritani Kohei   入谷　康平    所属   工学部 応用化学科    職種   助教
言語種別	英語
発行・発表の年月	2022/03
形態種別	学術論文
査読	査読あり
標題	Fabrication of cell plastics composed only of unicellular green alga
執筆形態	共著
掲載誌名	Appl. Microbiol.Biotechnol.
掲載区分	国外
出版社・発行元	Eueopw PMC
巻・号・頁	106,pp.4459-4468
担当区分	筆頭著者,責任著者
著者・共著者	Akihito Nakanishi, Kohei Iritani, Akane Tsuruta, Naotaka Yamamoto, Marina Watanabe, Nanami Ozawa, Masahiko Watanabe, Kuan Zhang, Ayaka Tokudome
概要	Cell plastics in this study were fabricated with only unicellular green alga Chlamydomonas reinhardtii as raw materials. The sizes of cell-major axis as structures were 8.4±1.2 μm, and the aspect ratios of those were 1.2±0.1, showing homogeneous particle size. After optimizing extraction condition of intracellular contents, cell plastics were fabricated with the cells as ingredient components and the intracellular contents as matrix components. Those cell plastics were observed with scanning electron microscopy, displaying the smooth surfaces of the cell plastics at a low magnifcation level. However, the surface, especially exposed surface, were rough at high magnifcation level. Tensile strength test revealed that increasing the ratio of intracellular contents in the cell plastics until 21% led enhancing mechanical properties of Young’s modulus and tensile strength; however, 25% of intracellular contents displayed decreases of those properties. As the optimal point, the cell plastic (21%), which contained 21% (w/w) of intracellular contents in cell plastics, showed 764±100 MPa and 8.6±5.2 MPa of Young’s modulus and tensile strength. The cell plastics showed few plastic region and soon fractured, indicating the possibility that cells and intracellular contents could be electrostatically connected. Additionally, cells were shown as a negative charge and displayed the possibility to contribute electrically cell-gathering with intracellular ionic components. Therefore, cells and intracellular contents containing ionic metabolites could be electrostatically connected for giving the mechanical strength to cell plastics. In this study, we successfully demonstrated fabricating cell plastics with only cells for the frst time and also showed the high possibility of conjugating each cell with the intracellular contents.