キムラ　マサヒロ   木村　将大    所属   応用生物学部 応用生物学科    職種   助教
言語種別	英語
発行・発表の年月	2019/08
形態種別	学術論文
査読	査読あり
標題	Direct comparison of chitinolytic properties and determination of combinatory effects of mouse chitotriosidase and acidic mammalian chitinase.
執筆形態	共著
掲載誌名	Int J Biol Macromol
掲載区分	国外
出版社・発行元	Elsevier社
巻・号・頁	134,pp.882-890
著者・共著者	Kimura, M., Umeyama, T., Wakita, S., Okawa, K., Sakaguchi, M., Matoska, V., Bauer, PO., and Oyama, Oyama, F.
概要	Chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase) have been implicated in food processing and various pathophysiological conditions such as chronic inflammatory diseases. By combination of the colorimetric analysis and fluorophore-assisted carbohydrate electrophoresis (FACE) method, we directly compared the chitinolytic properties of mouse Chit1 and AMCase and determined their combinatory effects in artificial and natural chitin substrates processing. Chit1 and AMCase display different dynamics of chitinolytic properties through acidic to neutral conditions. At pH2.0, the activity of AMCase was higher than that of Chit1 and stronger or comparable with that of Serratia marcescens chitinase B, a well-characterized bacterium chitinase. Changes of degradation products using different substrates indicate that AMCase and Chit1 have diverse properties under various pH conditions. Exposure of the chitin substrates to both Chit1 and AMCase did not indicate any mutual interference of these enzymes and showed no synergistic effect, in contrast to observations regarding some bacterial chitinases. Our results suggest that Chit1 and AMCase have no synergistic effect under physiological conditions.
外部リンクURL	https://www.sciencedirect.com/science/article/pii/S0141813019318008?via%3Dihub