キムラ　マサヒロ   木村　将大    所属   応用生物学部 応用生物学科    職種   助教
言語種別	英語
発行・発表の年月	2020/08
形態種別	学術論文
標題	Characterization of mouse di- N-acetylchitobiase that can degrade chitin-oligosaccharides
執筆形態	共著
掲載誌名	Biotechnol Biochem
掲載区分	国外
巻・号・頁	84,pp.2499-2507
著者・共著者	Ohno, M., Miyazaki, M., Kimura M, Minowa, Y., Sakaguchi, M., Oyama, F., Yamashita, T.
概要	Di-N-acetylchitobiase (Ctbs) degrades β-1,4 glycoside bonds of the chitobiose core of free asparagine-linked glycan. This study examined whether Ctbs degrades chitin-oligosaccharides to GlcNAc in mammals. We analyzed Ctbs mRNA and protein expression in mouse tissues and characterized enzymatic activity using recombinant mouse Ctbs expressed in Escherichia coli. Ctbs mRNA and protein were expressed in various tissues of mouse, including the stomach. Optimal conditions for recombinant Ctbs were pH 3.0 and 45°C, and the recombinant enzyme was retained more than 94% activity after incubation at pH 3.0-7.0 and below 37°C. The recombinant Ctbs hydrolyzed (GlcNAc)3 and (GlcNAc)6 at pH 3.0 and produced GlcNAc. The K m of Ctbs was lowest with (GlcNAc)3 as a substrate. k cat/K m was fourfold as high with (GlcNAc)3 and (GlcNAc)4 as substrates than with (GlcNAc)2. These results suggest that Ctbs digests chitin-oligosaccharides or (GlcNAc)2 of reducing-end residues of oligosaccharides and produces GlcNAc in mouse tissues.
外部リンクURL	https://academic.oup.com/bbb/article-abstract/84/12/2499/6044116?redirectedFrom=fulltext&login=true