アラカワ　タカヒロ   荒川　貴博    所属   工学部 電気電子工学科    職種   教授
言語種別	英語
発行・発表の年月	2025/03
形態種別	国際会議論文
査読	査読あり
標題	Non-invasive Lactate Monitoring System Using Mobile Imaging and Paper-Based Biosensors
執筆形態	共著
掲載誌名	Microactuators, Microsensors and Micromechanisms
掲載区分	国外
出版社・発行元	Springer Nature
巻・号・頁	117,pp.114-123
総ページ数	10
担当区分	最終著者,責任著者
著者・共著者	Ishi K, Ohnishi K, Chimoto T, Arakwa T.
概要	Lactate concentration in blood is utilized as an indicator of tissue oxygen metabolism for assessing the therapeutic effectiveness in the treatment of sepsis. A biosensor capable of non-invasively measuring lactate produced from metabolism is in demand. The ratio of sweat lactate to blood lactate correlates strongly with the lactate threshold, the use of sweat lactate is expected to provide a non-invasive measurement. In this study, a mobile imaging system and a paper-based biosensor were developed for real-time monitoring of lactate levels in living organisms. First, a paraffin-processed channel was created on the chromatography paper using a stamp. Next, we introduce a mobile device that combines UV-LEDs and bandpass filters for high stability. We constructed an imaging system that can quantify NADH concentrations ranging from 50 μmol/L to 1 mmol/L Finally, lactate dehydrogenase (LDH) and NAD+ were dropped into the paper stream for fluorescence imaging of lactate and quantitative characterization of lactate. Fluorescence of NADH generated by LDH was confirmed, suggesting the possibility of quantifying lactate.
外部リンクURL	https://doi.org/10.1007/978-3-031-83357-1_12