アラカワ　タカヒロ   荒川　貴博    所属   工学部 電気電子工学科    職種   准教授
言語種別	日本語
発行・発表の年月	2024/07
形態種別	学術論文
査読	査読あり
標題	Image Sensing of Gaseous Acetone Using Secondary Alcohol Dehydrogenase-Immobilized Mesh for Exhaled Air
執筆形態	共著
掲載誌名	Analytical Chemistry
掲載区分	国外
出版社・発行元	American Chemical Society
巻・号・頁	96(28),11549-11556頁
総ページ数	8
著者・共著者	Iitani K, Suzuki M, Ichikawa K, Toma K, Arakawa T, Mitsubayashi K
概要	Human-borne acetone is a potent marker of lipid metabolism. Here, an enzyme immobilization method for secondary alcohol dehydrogenase (S-ADH), which is suitable for highly sensitive and selective biosensing of acetone, was developed, and then its applicability was demonstrated for spatiotemporal imaging of concentration distribution. After various investigations, S-ADH-immobilized meshes could be prepared with less than 5% variation by cross-linking S-ADH with glutaraldehyde on a cotton mesh at 40 °C for 15 min. Furthermore, high activity was obtained by adjusting the concentration of the coenzyme nicotinamide adenine dinucleotide (NADH) solution added to the S-ADH-immobilized mesh to 500 μM and the solvent to a potassium phosphate buffer solution at pH 6.5. The gas imaging system using the S-ADH-immobilized mesh was able to image the decrease in NADH fluorescence (ex 340 nm, fl 490 nm) caused by the catalytic reaction of S-ADH and the acetone distribution in the concentration range of 0.1–10 ppm-v, including the breath concentration of healthy people at rest. The exhaled breath of two healthy subjects at 6 h of fasting was quantified as 377 and 673 ppb-v, which were consistent with the values quantified by gas chromatography–mass spectrometry.
外部リンクURL	https://doi.org/10.1021/acs.analchem.4c02251