アラカワ　タカヒロ   荒川　貴博    所属   工学部 電気電子工学科    職種   准教授
言語種別	英語
発行・発表の年月	2024/08
形態種別	学術論文
査読	査読あり
標題	Long-range surface plasmon hydrogel aptasensor for sensitive, selective, and continuous measurement of vancomycin
執筆形態	共著
掲載誌名	Sensors and Actuators B: Chemical
掲載区分	国外
出版社・発行元	Elsevier
巻・号・頁	413(15),pp.135882
著者・共著者	Y. Taguchi, K. Toma, K. Iitani, T. Arakawa, Y. Iwasaki, K. Mitsubayashi
概要	This research presents a long-range surface plasmon (LRSP) hydrogel aptasensor designed for precise detection and real-time monitoring of vancomycin (VCM), an antibiotic with a narrow therapeutic range. The aptasensor utilizes a three-dimensional binding matrix of 2-methacryloyloxyethyl phosphorylcholine (MPC) and N-methacryloyl-(L)-tyrosinemethylester (MAT) hydrogel for aptamer immobilization, yielding enhanced sensing capabilities. The hydrogel is coated on Au surface, gelled, and irradiated with light to immobilize peptide aptamers with VCM recognition sites. When exposed to VCM, the sensor displays changes in reflectivity proportional to the VCM concentration, with a dynamic range of 0.06–100 μM. The sensor demonstrated a 13-fold increase in sensitivity compared to an LRSP aptasensor without the hydrogel and showed high selectivity for VCM and high reproducibility with a coefficient of variation of 6.5% across 10 repeated measurements. Furthermore, when the VCM concentration was continuously varied without rinsing, a rapid change in reflectivity and output corresponding to changes in concentration was obtained, suggesting the possibility of real-time monitoring of VCM concentration.
外部リンクURL	https://doi.org/10.1016/j.snb.2024.135882