サトウ アツシ   Atsushi Sato   佐藤 淳    所属   応用生物学部 応用生物学科    職種   教授
言語種別	英語
発行・発表の年月	2025/08
形態種別	学術講演予稿集（学会、研究会を含む）
標題	Albumin-fused human lactoferrin inhibits lung cancer cell migration by impaired Na+/H+ exchanger 7-driven organelle pH
執筆形態	共著
掲載誌名	ラクトフェリン2025
掲載区分	国内
出版社・発行元	アイ・ケイ　コーポレーション
担当区分	責任著者
著者・共著者	Nopia Hana, Masahiro Kimura, and Atsushi Sato
概要	The fusion of human serum albumin (HSA) to human lactoferrin (hLF) (here in referred to as hLF-HSA) has strongly exhibited anti-migratory effects through the downregulation of matrix metalloprotease 1 (MMP1) against the human lung adenocarcinoma PC-14 cells. In this study, we report that the upregulation of Na+/H+ exchanger 7 (NHE7) induced by hLF-HSA corresponds to its anti-migratory effects. hLF-HSA showed organelle alkalization via NHE7 upregulation in PC-14 cells, disrupting the organelle pH homeostatic. This disruption of organelle pH homeostasis, particularly in the trans-Golgi network (TGN), leads to the downregulation of MMP1, a key molecule in the anti-migratory activity of hLF-HSA, thereby reducing migration. Moreover, hLF-HSA-induced downregulation of MMP1 subsequently reversed the epithelial-mesenchymal transition (EMT), a process that can promote tumor-initiating potential during cancer metastasis. The reverse process of EMT suppresses PC-14 cell migration. Therefore, hLF-HSA-induced organelle pH dysfunction in cancer cells represents a novel and promising approach to suppressing cancer migration. Further studies on the versatility of hLF-HSA across various cancer cell types are necessary to fully establish its potential as an anti-tumor drug candidate.
外部リンクURL	https://lactoferrin.jp/archive.html