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言語種別	英語
発行・発表の年月	2024/02
形態種別	学術論文
査読	査読あり
標題	Evaluation of Cellular Responses of Heterotrophic Escherichia coli Cultured with Autotrophic Chlamydomonas reinhardtii as a Nutrient Source by Analyses Based on Microbiology and Transcriptome
執筆形態	共著
掲載誌名	Microorganisms
掲載区分	国外
出版社・発行元	MDPI
巻・号・頁	12(3),pp.452
著者・共著者	Akihito Nakanishi, Natsumi Omino, Tomoyo Nakamura, Saki Goto, Riri Matsumoto, Misaki Yomogita, Naoki Narisawa, Manami Kimijima and Kohei Iritani
概要	The substance-producing strain Escherichia coli LS5218 was co-cultured with the freshwater green alga Chlamydomonas reinhardtii C-9: NIES-2235, and its cellular responses were evaluated using microbial methods and transcriptomic analysis. Even when using the green alga as a nutrient source, E. coli exhibited a growth rate comparable to that in Luria–Bertani medium. Transcriptomic analysis revealed upregulation of genes related to glycolysis and lipid synthesis, while genes involved in lipid degradation were downregulated. These responses suggest carbon source assimilation, indicating that E. coli can directly utilize Chlamydomonas reinhardtii as a nutrient supplier.
DOI	10.3390/microorganisms12030452
外部リンクURL	https://doi.org/10.3390/microorganisms12030452