ナカムラ　ヒデアキ   Hideaki Nakamura   中村　秀明    所属   学環 教養学環    職種   専任講師
言語種別	英語
発行・発表の年月	2024
形態種別	学術論文
招待論文	招待あり
標題	"A chemiluminometric inorganic pyrophosphate ion assay using inorganic pyrophosphatase, pyruvate oxidase, and isoluminol for specific detection of E. coli RNA" in press
執筆形態	共著
掲載誌名	Current Topics in Analytical Chemistry
掲載区分	国外
出版社・発行元	Research Trends Ltd.
担当区分	筆頭著者,責任著者
著者・共著者	Hideaki Nakamura,*, Noriko Suzuki, Sachie Okubo, Koji Karasawa, Masaru Kato and Hidetoshi Arakawa
概要	In the present study, we tried to establish a new chemiluminometric inorganic pyrophosphate ion (CL-PPi) assay method by combining the advantages of the assay techniques that have been studied in each laboratory of Arakawa and Nakamura laboratories. Utilizing the CL-PPi assay, we attempted E. coli RNA (species-specific sequence in the 16S rRNA) detection through the NASBA reaction. In the CL-PPi assay, enzymatic IP-PO reactions employed for PPi catalysis and H2O2 production, H2O2 was subsequently detected by the CL reaction of isoluminol and m-POD.